Coronavirus information from UCLA and UCLA Health.

Molecular Screening Shared Resource

The MSSR provides a comprehensive range of high-throughput screening (HTS) services including chemical genomics, functional genomics, and drug discovery.

COVID-19 Info

We have the following materials available for COVID-19 research:

CRISPR Library-dual sgRNA: pCLIP-dual-SFFV-ZsGreen vector backbone, GFP visualization tag
5 Vectors of ACE2 (10 total sgRNA’s)
2 Vectors of TMPRSS2 (4 total sgRNA’s)

cDNA Library
Ace2-BC026801-pCMV-Sport6 (pSport6) {*MOUSE*}
ACE2-BC048094-pBluescriptR
ACE2-BC039902-pSport6 -*currently cloning into pLX304 and pB_TAC_ERP2 (tet-inducible)*
Tmprss2-BC038393-pSport6 {*MOUSE*}
TMPRSS2-BC051839-pSport6 (TOLH Library-1507307)
TMPRSS2-BC051839-pSport6 (TOLH Library-1514871)
TMPRSS2-BC051839-pSport6 (IRAK Library)

shRNA Library-pGIPZ vector-GFP Tag
ACE2-x3 Vectors
TMPRSS2- x5 Vectors

siRNA Library
ACE2- x4 Constructs

Pseudovirus

Cell Lines
HEK293t: ACE2 KO, ACE2 OE,TMPRRS2 KO, TMPRRS2 OE
HeLa: ACE2 KO, ACE2 OE, TMPRSS2 KO, TMPRSS2 OE
ExpiCho: ACE2-FC-FLAG