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Molecular Screening Shared Resource

Delivering High Throughput Screening to the UCLA Research Community

High-Throughput Screening | Drug Discovery | Functional Genomics

The MSSR has automated equipment and data collection that make it possible to test about 30,000 compounds (or more) on a system of interest in about a day

Molecular Screening Shared Resource

The MSSR provides a comprehensive range of high-throughput screening (HTS) services including chemical genomics, functional genomics, and drug discovery.

Services

For compounds screens, data points are in singlets. For functional genomics screens we use duplicates. We typically utilize 384 well plates but are flexible in this regard. We can accomodate any plate type ranging from 96 to 1536 well plates. Specialty plates can be handled as well on an as-needed base. We have special rates for consumables with many vendors based on the volume we use. These reduced rates are passed on directly to our screeners.

Please do not let the cost of screening deter you from discussing your assay and interest with MSSR personnel. Limited funds may be available for proposed screens.

Hours of operation: The facility is typically open for consultation from 9:00 am-5:00 pm, Monday through Friday. Screens may begin earlier and run longer than this allotted time as needed.

Assay Development

Internal Rate: $3082.00

JCCC Rate: $582.55

External Rate: $3082.00

Small Molecule Screening (up to 100K)

Internal Rate: $2684.00

JCCC Rate: $507.33

External Rate: $2684.00

Small Molecule Screening (additional 100K)

Internal Rate: $3771.00

JCCC Rate: $712.79

External Rate: $3771.00

CRISPR

CRISPR knockout libraries are designed to create insertions or deletions in targeted genes across the genome. Our libraries are designed for targets throughout the genome. CRISPR activation libraries use gRNAs to guide dCas9 bound to a transcriptional activator to target genes and thereby activate their expression. CRISPR repression libraries use gRNAs to guide dCas9 bound to a transcriptional repressor to target genes, block transcription initiation, and thereby repress, or knockdown, their expression.

We can also design gRNAs specific to your gene of interest. Our gRNAs are designed and validated to work with our genome editing tools to provide consistent high efficiency editing. We use high performance oligo manufacturing to design our guides. Whether you need an economical solution for routine editing tasks or you want to drive maximum editing efficiency, particularly in primary or stem cells, we have the tools required to introduce your specific edit in your cell line.

Access to clones (cDNA)

Internal Rate: $59.00

JCCC Rate: $11.15

External Rate: $59.00

Request Clones

Full Genome cDNA Screening

Internal Rate: $2813.00

JCCC Rate: $531.71

External Rate: $2813.00

shRNA Screening (human, per sub-library)

Internal Rate: $2963.00

JCCC Rate: $560.06

External Rate: $2963.00

siRNA Screening (human, genome wide)

Internal Rate: $2963.00

JCCC Rate: $560.06

External Rate: $2963.00

siRNA Screening (mouse, genome wide)

Internal Rate: $2963.00

JCCC Rate: $560.06

External Rate: $2963.00

Full Technical Support (per hour)

Internal Rate: $32.39

JCCC Rate: $32.36

External Rate: $32.39

Data Analysis (per hour)

Internal Rate: $155.00

JCCC Rate: $29.30

External Rate: $155.00